A simple, rapid, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed for the estimation of methylcobalamin in bulk and solid dosage form. The determination was carried out on a C-8 (250 × 4.6 mm, 5 micron) column using mobile phase 1.0 %w/v solution of sodium dihydrogen phosphate dihydrate and acetonitrile in the ratio of 735:265 adjusts pH 3.50 with 10% ortho phosphoric acid and then added 3.76 gm/liter of sodium 1-hexane sulfonate (hexane-1-sulfonic acid sodium salt). The flow rate and runtime were 1 ml/min and 10 min, respectively. The eluent was monitored at 318 nm. The retention time of methylcobalamin was 2.88 mins. The method was reproducible, the detector response was found to be linear in the concentration range of 16 ppm to 24 ppm.
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